Phasor analysis of multiphoton spectral images distinguishes autofluorescence components of in vivo human skin |
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| Authors: | Farzad Fereidouni Arjen N Bader Anne Colonna Hans C Gerritsen |
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| Institution: | 1. Utrecht University, Department of Molecular Biophysics, Utrecht, The Netherlands;2. Wageningen University, Laboratory of Biophysics, Microspectroscopy Centre, Wageningen, The Netherlands;3. L'Oréal Recherche et Innovation, Aulnay‐sous‐Bois, France |
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| Abstract: | Skin contains many autofluorescent components that can be studied using spectral imaging. We employed a spectral phasor method to analyse two photon excited autofluorescence and second harmonic generation images of in vivo human skin. This method allows segmentation of images based on spectral features. Various structures in the skin could be distinguished, including Stratum Corneum, epidermal cells and dermis. The spectral phasor analysis allowed investigation of their fluorescence composition and identification of signals from NADH, keratin, FAD, melanin, collagen and elastin. Interestingly, two populations of epidermal cells could be distinguished with different melanin content. (© 2014 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) |
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| Keywords: | fluorescence microscopy multispectral and hyper spectral imaging image analysis spectral unmixing tissue imaging human skin |
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