Mitochondrial resistance to miconazole in Saccharomyces cerevisiae |
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Authors: | Francisco Portillo and Carlos Gancedo |
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Affiliation: | (1) Departmento de Bioquímica, Facultad de Medicina UAM, Instituto de Investigaciones Biomédicas del CSIC (Enzimología, Arzobispo Morcillo, 4. E-28029 Madrid, Spain |
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Abstract: | Summary One mutant of mitochondrial origin resistant to miconazole has been isolated and characterized in S. cerevisiae. The mutation is linked to the locus oli1, the structural gene for subunit 9 of ATPase on mitochondrial DNA. Miconazole inhibited the mitochondrial ATPase of the wild type while the enzyme of the resistant mutant was insensitive to this effect. Levels of ATP decreased to one-third of the control in the wild type in the presence of miconazole, while they were unaffected in the mutant.Abbreviations MNNG N-methyl-N-nitrosoguanidine - Mics/Micr phenotypic sensitivity/resistance to miconazole - M1R mitochondrial locus conferring miconazole resistance - rho+/rho- grand/cytoplasmic petite - rhoo cytoplasmic petite deleted of all mitochondrial DNA - w+ mitochondrial locus conferring polarity of recombination |
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