Standardization of an indirect PTA-ELISA for detection ofFusarium spp. in infected grains |
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Authors: | S Rohde F Rabenstein |
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Institution: | (1) Bundesanstalt für Züchtungsforschung an Kulturpflanzen, Institut für Resistenzforschung und Pathogendiagnostik, Postfach 1505, D-06435 Aschersleben |
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Abstract: | Plant pathogenic fungi of the genusFusarium cause agriculturally important diseases of small grain cereals and maize. Especially the contamination of grains with the
mycotoxin deoxynivalenol (DON) is harmful for animals and humans. For quantification of the severity ofFusarium head blight (FHB) in resistance evaluation and selection of cereals a fast, economical and reliable method is essential.
Immunological methods appear to be particularly suitable for such an assessment. The results demonstrate that a selected antiserum
was appropriate for the detection ofFusarium-exoantigens (ExoAg) in cereal grains by an indirect ELISA-format, although a discrimination between variousFusarium-species was not possible. The polyclonal antibody detection system was optimized for different parameters and a standard
test protocol was elaborated.
Presented at the 26th Mykotoxin-Workshop in Herrsching, Germany, May 17–19, 2004 |
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Keywords: | immunoassay Fusarium spp test procedure wheat resistance evaluation |
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