A novel biotinylated diazirinyl ceramide analogue for photoaffinity labeling |
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| Authors: | Hashimoto Makoto Hatanaka Yasumaru |
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| Institution: | Department of Agricultural and Life Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Hokkaido, Japan. hasimoto@obihiro.ac.jp |
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| Abstract: | A novel photoreactive ceramide analogue, which contains (3-trifluoromethyl)phenyldiazirinyl lipid and biotinylated sphingosine, was synthesized. The probe was recognized as an antigen by anti-ceramide antibody and as a substrate for sphingolipid ceramide N-deacylase. These results indicate that the probe may be useful as a photoaffinity-biotinylating agent in sphingolipid studies. |
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