Elongation factor G and protein S12 are the nearest neighbours in the Escherichia coli ribosome |
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Authors: | Alexander S. Girshovich Elena S. Bochkareva Yuri A. Ovchinnikov |
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Affiliation: | Institute of Protein Research Academy of Sciences of the U.S.S.R. Poustchino, Moscow Region, U.S.S.R. |
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Abstract: | It has been found that iodine oxidation of the complex of ribosomes with EF-G2 results in the formation of a disulphide bond between the single exposed sulphydryl group of the factor and each of the ribosomal subunits. It is concluded that this EF-G region is localized on the ribosomal interface.Protein S12 is identified as the ribosomal component crosslinked with EF-G in the oxidation reaction. The possible role of the specific interaction of EF-G with protein S12 for the functioning of the factor as an activator of translocation is discussed. |
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