Abstract: | Crystals of calotropin DI (Mr 23,400), have been prepared by microdialysis against 5% (w/v) polyethylene glycol 20,000 in water, pH 7.0. They have orthorhombic space group P212121 with cell parameters . Crystals of calotropin DII (Mr 24,000), prepared by the same technique against 5% (w/v) polyethylene glycol 20,000 in phosphate buffer of low ionic strength, pH 7.0, display monoclinic space group C2 with cell parameters . In both cases, there is only one molecule in the asymmetric unit. |