| 纤毛虫大核提取方法上的改进及其扫描电镜观察 |
| |
| 引用本文： | 顾立刚,赵柳,顾福康. 纤毛虫大核提取方法上的改进及其扫描电镜观察[J]. 动物学杂志, 2005, 40(3): 57-59 |
| |
| 作者姓名： | 顾立刚 赵柳 顾福康 |
| |
| 作者单位： | 华东师范大学生命科学学院,上海,200062;华东师范大学生命科学学院,上海,200062;华东师范大学生命科学学院,上海,200062 |
| |
| 摘 要： | 以包囊游仆虫(Euplotes encysticus)为材料，对Arikawa等报道的纤毛虫大核提取方法进行了改进，提出了一种更为简便有效的方法，即：用去垢剂Triton X-100处理细胞，结合玻璃微吸管的物理破碎，得到完整的大核后，应用扫描电镜观察。
|
| 关 键 词： | 纤毛虫 大核 提取 扫描电镜 |
| 文章编号： | 0250-3263(2005)03-57-03 |
An Improved Method of Macronuclear Isolation of Ciliates for Scanning Electron Microscopy |
| |
| GU Li-gang,ZHAO Liu,GU Fu-kang. An Improved Method of Macronuclear Isolation of Ciliates for Scanning Electron Microscopy[J]. Chinese Journal of Zoology, 2005, 40(3): 57-59 |
| |
| Authors: | GU Li-gang ZHAO Liu GU Fu-kang |
| |
| Abstract: | A more simplified and effective method is employed to isolate the macronuclei from ciliates and to prepare sample for scanning electron microscopy, based on the procedure described by Arikawa. Two main steps of the improved method are to treat the cells with Triton X-100 and to lysis them with tiny glass tubes. |
| |
| Keywords: | Ciliates Macronucleus Isolation Scanning electron microscopy |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《动物学杂志》浏览原始摘要信息 |
|
点击此处可从《动物学杂志》下载全文 |
