老年人胃内菌群研究

目的研究人体胃内菌群,探讨老年与非老年人胃内菌群的差异。方法选择67例无严重胃肠道疾病的患者为研究对象。其中男性50例、女性17例。年龄≥60岁53例,〈60岁14例。胃镜下取胃组织及胃液,测胃液pH,并做胃组织需氧、厌氧细菌培养及真菌培养,计数胃组织细菌培养数量。16SrRNA法鉴定胃组织细菌种类。真菌的鉴定按微生物科常规菌种方法鉴定。结果老年人中胃内需氧细菌培养阳性为23例（48.93%）,12例（25.53%）胃内需氧菌培养〉1×10^5CFU/g;厌氧细菌培养阳性为22例（46.81%）,12例（25.53%）胃内厌氧菌培养〉1×10^5CFU/g。非老年人中需氧细菌培养阳性为4例（28.57%）,1例（7.14%）胃内需氧菌培养〉1×10^5CFU/g;厌氧细菌培养阳性为4例（28.57%）,1例（7.14%）胃内厌氧菌培养〉1×10^5CFU/g。但老年人与非老年人比较,细菌培养阳性率及细菌培养〉1×10^5CFU/g的比率差异无显著性。仅1例老年人胃组织分离出真菌,为白色念珠菌。胃内共分离出细菌69株,其中革兰阳性球菌31株（44.93%）,革兰阳性杆菌12株（17.39%）,革兰阴性球菌11株（15.94%）,革兰阴性杆菌15株（21.74%）。需氧菌13株（18.84%）,需氧兼性厌氧菌54株（78.26%）,专性厌氧菌2株（2.90%）。老年人胃内常见的细菌是：链球菌、大肠埃希菌、奈瑟菌;非老年人胃内常见的细菌是：链球菌和大肠埃希菌。多为口咽部和胃肠道常见菌群,部分为条件致病菌。结论约46%～48%的老年人胃内细菌培养阳性,约25%的老年人有胃内细菌过度生长（〉1×10^5CFU/g）。约28%的非老年人胃内细菌培养阳性,约7%的非老年人有胃内细菌过度生长。老年人胃内菌群分布与非老年人相似,为口咽部和胃肠道常见菌群,部分为条件致病菌。

The approach to human gastric microflora

Objective To investigate the human gastric microbial population, and approach the difference between senile and young people. Method 67 cases without severe gastrointestinal disease were selected in this research, 50 male, 17 female, 53 cases ≥60yr. We got gastric fluid and gastric tissue from gastroscope, and measured the pH value of gastric fluid. Aerobic and anoxybiontic bacteria culture together with fungal culture of gastric tissue were performed. Then we counted the bacteria number. The means of 16S rRNA was used to identify the bacteria of gastric tissue. Fungal identification was done by routine method. Result Among the senile, 23(48.93%) cases were positive of aerobic bacteria culture, 12(25.53%)cases were >1 × 10~5 CFU/g, and 22 cases were positive of anaerobic bacteria culture, 12(25.53%) cases were >1 × l0~5 CFU/g. Among the young, 4(28. 57%) cases were positive of aerobic bacteria culture, 1(7.14%) case was >1 × 10~5 CFU/g, and4(28.57%) cases were positive of anaerobic bacteria culture, 1(7.14%) case was >1 × 10~5 CFU/g. But, there was not statistical significance between senile and young group in number of bacteria culture. Only in 1 case could isolate fungi in senile group, it was Candida albicans. 69 bacteria strains were isolated from stomach. There were 31 strains(44.93%) Gram-positive cocci, 12 strains(17.39%) Gram-positive bacilli, 11 strains(15.94%) Gram-negative cocci, and 15 strains(21.74%) Gram-negative bacilli. 13 strains(18.84%)were aerobic, 2 strains (2.90%) were anaerobic, and 54 strains (78.26%) were both aerobic and anaerobic. Among the senile people, the common types were coli. Conclusion About 46%～48% of senile are positive of bacteria culture, about 25% are bacteria overgrowth. About 28% of young are positive of bacteria culture, about 7% are bacteria overgrowth. The gastric microflora is similar among senile and young people. They are the common bacteria in oropharynx and gastrointestinal tract, some are opportunistic bacteria. Streptococcus, Escherichia coli and Neisseria. Among the young, the common types were streptococcus and Escherichia