Enhanced degradation of gamma-irradiated lignocelluloses by a new xylanolytic Flavobacterium sp. isolated from soil |
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Authors: | Arvind Kumar Bhatt Tek Chand Bhalla Hari Om Agrawal N Sharma |
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Institution: | Microbiology and Sanitation Discipline, Central Food Technological Research Institute, Mysore-570 013, Karnataka State, India |
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Abstract: | An extracellular chitosanase produced by Rhodotorula gracilis CFR-1 that catalyses a limited degradation of chitosan with no detectable generation of glucosamine or reducing groups was identified. Ultracentrifugation, polyacrylamide gel electrophoresis and gel permeation studies suggest that chitosan of average molecular mass 36000 Da was reduced by the enzymic catalysis to nearly one-fourth this size without further hydrolysis of the products. The enzyme, produced constitutively by this yeast, was partially purified and some of its properties were studied. |
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