Genetic transformation of Cavendish banana (Musa spp. AAA group) cv 'Grand Nain' via microprojectile bombardment |
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| Authors: | D K Becker B Dugdale M K Smith R M Harding J L Dale |
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| Institution: | (1) Centre for Molecular Biotechnology, School of Life Sciences, Queensland University of Technology, GPO Box 2434, Brisbane, Queensland 4001, Australia e-mail: j.dale@qut.edu.au Fax: (+61) 7 3864 1534, AU;(2) Maroochy Research Station, Queensland Horticulture Institute, Department of Primary Industries, Queensland, Australia, AU |
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| Abstract: | An effective method has been developed for the stable transformation and regeneration of Cavendish banana (Musa spp. AAA group) cv 'Grand Nain' by microprojectile bombardment. Embryogenic cell suspensions were initiated using immature
male flowers as the explant. Cells were co-bombarded with the neomycin phosphotransferase (nptII) selectable marker gene under the control of a banana bunchy top virus (BBTV) promoter or the CaMV 35S promoter, and either
the β-glucuronidase (uidA) reporter gene or BBTV genes under the control of the maize polyubiquitin promoter. Plants were regenerated, under selection
with kanamycin, that were co-transformed with nptII and either the uidA or BBTV genes. Molecular characterisation of transformants demonstrated that the transgenes had been stably integrated into
the banana genome.
Received: 22 June 1998 / Revision received: 29 March 1999 / Accepted 1 May 1999 |
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| Keywords: | Musa Banana Transformation Microprojectile bombardment |
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