A sensitive radioimmuno assay for thymine dimers |
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Authors: | Helmut Klocker Bernhard Auer Helmut J. Burtscher Johann Hofmann Monica Hirsch-Kauffmann Manfred Schweiger |
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Affiliation: | (1) Institut für Biochemie (nat. Fak.), Universitaet Innsbruck, A-6020, Austria |
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Abstract: | Summary A sensitive radioimmuno assay (RIA) method for detection of the UV photoproduct, thymine dimers has been developed. The limit of detection of this method is 6×10-14 mol or 15 pg thymine dimer. It is highly specific: A structurally similar compound such as uridine dimer interferes with the detection of thymine dimers only when it is 53,000-fold or more in molar excess. Since this RIA method does not require the use of labeled DNA, it represents a considerable improvement for repair studies with radiation-sensitive cells. |
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