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A sensitive radioimmuno assay for thymine dimers

Authors:	Helmut Klocker Bernhard Auer Helmut J Burtscher Johann Hofmann Monica Hirsch-Kauffmann and Manfred Schweiger

Institution:	(1) Institut für Biochemie (nat. Fak.), Universitaet Innsbruck, A-6020, Austria

Abstract:	Summary A sensitive radioimmuno assay (RIA) method for detection of the UV photoproduct, thymine dimers $\left( {\overline {TT} } \right)$ has been developed. The limit of detection of this method is 6×10^-14 mol or 15 pg thymine dimer. It is highly specific: A structurally similar compound such as uridine dimer $\left( {\overline {UU} } \right)$ interferes with the detection of thymine dimers only when it is 53,000-fold or more in molar excess. Since this RIA method does not require the use of labeled DNA, it represents a considerable improvement for repair studies with radiation-sensitive cells.

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