Euphorbiasteroid,a component of Euphorbia lathyris L., inhibits adipogenesis of 3T3‐L1 cells via activation of AMP‐activated protein kinase |
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| Authors: | Su‐Jin Park Jae Ho Park Anna Han Munkhtugs Davaatseren Hyun Jin Kim Myung‐Sunny Kim Haeng Jeon Hur Mi‐Jeong Sung Jin‐Taek Hwang Hye Jeong Yang Dae Young Kwon |
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| Affiliation: | 1. Korea Food Research Institute, Division of Nutrition And Metabolism Research, Seongnam‐si, Korea;2. The University of Tennessee‐Knoxville, Department of Nutrition, College of Education, Health & Human Sciences, Knoxville, TN, USA;3. Konkuk University, Department of Bioresources and Food Science, College of Life and Environmental Sciences, Seoul, Gwangjin‐Gu, Korea;4. Gyeongsang National University, Department of Food Science & Technology, Jinju, Korea |
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| Abstract: | The purpose of this study is to investigate the effects of euphorbiasteroid, a component of Euphorbia lathyris L., on adipogenesis of 3T3‐L1 pre‐adipocytes and its underlying mechanisms. Euphorbiasteroid decreased differentiation of 3T3‐L1 cells via reduction of intracellular triglyceride (TG) accumulation at concentrations of 25 and 50 μM. In addition, euphorbiasteroid altered the key regulator proteins of adipogenesis in the early stage of adipocyte differentiation by increasing the phosphorylation of AMP‐activated protein kinase (AMPK) and acetyl‐CoA carboxylase. Subsequently, levels of adipogenic proteins, including fatty acid synthase, peroxisome proliferator‐activated receptor‐γ and CCAAT/enhancer‐binding protein α, were decreased by euphorbiasteroid treatment at the late stage of adipocyte differentiation. The anti‐adipogenic effect of euphorbiasteroid may be derived from inhibition of early stage of adipocyte differentiation. Taken together, euphorbiasteroid inhibits adipogenesis of 3T3‐L1 cells through activation of the AMPK pathway. Therefore, euphorbiasteroid and its source plant, E. lathyris L., could possibly be one of the fascinating anti‐obesity agent. Copyright © 2015 John Wiley & Sons, Ltd. |
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| Keywords: | euphorbiasteroid obesity adipogenesis AMPK 3T3‐L1 |
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