Hypothalamic energy metabolism is impaired by doxorubicin independently of inflammation in non‐tumour‐bearing rats |
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| Authors: | Barbara M M Antunes Fabio Santos Lira Gustavo Duarte Pimentel José Cesar Rosa Neto Andrea Maculano Esteves Lila Missae Oyama Cláudio Teodoro de Souza Cinara Ludvig Gonçalves Emilio Luiz Streck Bruno Rodrigues Ronaldo Vagner dos Santos Marco Túlio de Mello |
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| Institution: | 1. Exercise and Immunometabolism Research Group, Department of Physical Education, Universidade Estadual Paulista (UNESP), Presidente Prudente, SP, Brazil;2. Departamento de Clínica Médica, Faculdade de Ciências Médicas (FCM), Universidade do Estadual de Campinas, Campinas, S?o Paulo, Brazil;3. Immunometabolism Research Group, Institute of Biomedical Sciences, University of S?o Paulo, S?o Paulo, SP, Brazil;4. Faculdade de Ciências Aplicadas, Universidade Estadual de Campinas, Limeira, S?o Paulo, Brazil;5. Departamento de Fisiologia, Universidade Federal de S?o Paulo, S?o Paulo, Brazil;6. Laboratório de Fisiologia e Bioquímica do Exercício, Unidade de Ciências da Saúde, Universidade do Extremo Sul Catarinense, Criciúma, Santa Catarina, Brazil;7. Laboratório de Fisiopatologia Experimental, Universidade do Extremo Sul Catarinense, Santa Catarina, Brazil;8. Faculty of Physical Education, University of Campinas (FEF‐UNICAMP), S?o Paulo, Brazil;9. Departamento de Biociências, Universidade Federal de S?o Paulo, Santos, S?o Paulo, Brazil;10. Sport Psychology Laboratory, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil |
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| Abstract: | We sought to explore the effects of doxorubicin on inflammatory profiles and energy metabolism in the hypothalamus of rats. To investigate these effects, we formed two groups: a control (C) group and a Doxorubicin (DOXO) group. Sixteen rats were randomly assigned to either the control (C) or DOXO groups. The hypothalamus was collected. The levels of interleukin (IL)‐1β, IL‐6, IL‐10, TNF‐α and energy metabolism (malate dehydrogenase, complex I and III activities) were analysed in the hypothalamus. The DOXO group exhibited a decreased body weight (p < 0.01). Hypothalamic malate dehydrogenase activity was reduced when compared with control (p < 0.05). In addition, pro‐inflammatory cytokine levels were unchanged. Therefore, our results demonstrate that doxorubicin leads to an impairment of \hypothalamic energy metabolism, but do not affect the inflammatory pathway. Copyright © 2015 John Wiley & Sons, Ltd. - Introduction
- Material and Methods
- Results
- Discussion
- Conflict of Interest
Significance paragraph The hypothalamus is a central organ that regulates a great number of functions, such as food intake, temperature and energy expenditure, among others. Doxorubicin can lead to deep anorexia and metabolic chaos; thus, we observed the effect of this chemotherapeutic drug on the inflammation and metabolism in rats after the administration of doxorubicin in order to understand the central effect in the hypothalamus. Drug treatment by doxorubicin is used as a cancer therapy; however the use of this drug may cause harmful alterations to the metabolism. Thus, further investigations are needed on the impact of drug therapy over the long term. |
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| Keywords: | doxorubicin energy metabolism inflammation hypothalamus cancer |
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