RECONSTITUTION OF A MEMBRANE-LIKE STRUCTURE WITH STRUCTURAL PROTEINS AND LIPIDS ISOLATED FROM TOBACCO THYLAKOID MEMBRANES |
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| Authors: | Jeng-sheng Huang Pi-yu Huang Robert N. Goodman |
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| Affiliation: | Department of Plant Pathology, University of Missouri, Columbia |
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| Abstract: | Structural proteins and lipids were extracted from thylakoid membranes and were dissolved separately with sodium dodecyl sulfate. Dissolved structural proteins reaggregated into spherical clumps, and lipids into globules composed of alternating light and dark bands, upon dialysis against dilute beta-buffer containing 20 mM Mg++. The reaggregates of the mixture of structural proteins and lipids formed membranous structures. The appearance and thickness of the reconstituted membranes are similar to those of thylakoid membranes prepared from sonicated chloroplasts. |
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