IN VITRO CULTURE OF SOMATIC WHEAT CALLUS TISSUE |
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| Authors: | E J Trione L E Jones R J Metzger |
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| Institution: | Department of Botany and Plant Pathology and Department of Farm Crops, Oregon State University, Corvallis |
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| Abstract: | Sterile culture of excised wheat tissue, Triticum vulgare Vill., was attempted on 20 different media. Most tissue explants did not grow. Endosperm tissue did not grow on any of the media. Callus tissue from intercalary meristems grew for several weeks but gradually became necrotic. Callus tissue derived from the cotyledonary node grew well and produced branching and enlarging root systems when placed on different media. Shoot primordia were not observed. Callus tissue derived from 13 varieties of wheat was cultured. These unique somatic tissues are interesting experimental materials for a study of the developmental biology of wheat. |
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