GLUCOSE DISSIMILATION IN USTILAGO MAYDIS

Mc Kinsey , Richard D. (U. Virginia, Charlottesville.) Glucose dissimilation in Ustilago maydis. Amer. Jour. Bot. 46(8): 566–571. Illus. 1959.—Studies were made of the manner in which glucose is dissimilated in Ustilago maydis. Radioactive isotope experiments utilizing glucose-1-C¹⁴ and glucose-6-C¹⁴ gave a C6/C1 ratio of 0.23 indicating that the major portion of the glucose was being metabolized by a direct shunt mechanism. Spectrophotometric and manometric experiments indicated the probable presence of the following enzyme systems: aldolase, 3-phosphoglyceraldehyde dehydrogenase, phosphoglyceryl kinase, alcohol dehydrogenase, glucose-6-phosphate dehydrogenase, and 6-phosphogluconic acid dehydrogenase. Manometric and growth experiments indicated that Ustilago maydis is an obligate aerobe.