Restriction enzyme banding of mouse metaphase chromosomes |
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| Authors: | Margot Kaelbling Dorothy A. Miller Orlando J. Miller |
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| Affiliation: | (1) Department of Human Genetics and Development, Columbia University, 10032 New York, NY, USA;(2) Cancer Center, Columbia University, 10032 New York, NY, USA;(3) Department of Obstetrics and Gynecology, Columbia University, 10032 New York, NY, USA;(4) Present address: Department of Genetics, Albert Einstein College of Medicine, 10461 New York, NY, USA |
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| Abstract: | Fixed metaphase chromosomes from mouse strain RIII embryos or A9 cells were treated with a restriction endonuclease, followed by Giemsa staining. Aha I, Hinf I, or Mbo I treatment produced a C-band pattern, and Eco RII or Hae III produced a G-band plus C-band pattern. Ava II and Bst NI each produced a G-band pattern, but on most chromosomes only a small segment of each C-band, adjacent to the centromere, was stained. These tiny residual C-bands may contain a minor satellite located adjacent to the major satellite clusters. |
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