Characterization of a rat liver epithelial cell line to detect inhibitors of metabolic cooperation |
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Authors: | C Jone J E Trosko C C Chang |
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Institution: | (1) Department of Pediatrics and Human Development, Center of Environmental Toxicology, College of Human Medicine, Michigan State University, 48824 East Lansing, Michigan |
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Abstract: | Summary A normal rat liver epithelial cell line, with phenotype characteristics of “oval” cells (WB-F344), was examined for its ability
to perform gap-junctional intercellular communication as measured by metabolic cooperation. To test for gap-junctional intercellular
communication, 6-thioguanine-sensitive cells were cocultivated with 6-thioguanine-resistant cells. It was found that the recovery
of 6-thioguanine-resistant cells depended on the densities of the 6-thioguanine-sensitive cells. Higher densities of 6-thioguanine-sensitive
cells reduced the recovery of 6-thioguanine-resistant cells. These observations demonstrate that rat liver epithelial cells
could metabolically cooperate, implying they could perform gap-junctional intercellular communication. Two tumor-promoting
organochlorine pesticides, aldrin and dieldrin, were potent inhibitors of metabolic cooperation for these cells, but 12-0-tetradecanoyl-phorbol-13-acetate
and teleocidin, known mouse skin tumor promoters, were not significantly effective in inhibiting metabolic cooperation. The
results suggest that these cells might provide the basis for an in vitro assay specifically to study liver tumor promoters.
Research was sponsored by a grant from the Air Force Office of Scientific Research, Air Force Systems Command, USAF, under
grant AFOSR-86-0084. The U. S. Government is authorized to reproduce and distribute reprints for government purposes notwithstanding
any copyright notation thereon. |
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Keywords: | metabolic cooperation tumor promoters liver epithelial cells |
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