Oxygen-dependent xylitol metabolism in Pichia stipitis |
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Authors: | H Jeppsson K Holmgren B Hahn-Hägerdal |
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Institution: | (1) Department of Applied Microbiology, Center for Chemistry and Chemical Engineering, Lund Institute of Technology/Lund University, P.O. Box 124, S-221 00 Lund, Sweden e-mail: Barbel.Hahn-Hagerdal@tmb.lth.se Tel: +46-46-2228428 Fax: +46-46-2224203, SE |
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Abstract: | Pichia stipitis CBS 6054 was cultivated in chemostat cultures under aerobic and oxygen-limited conditions with xylitol alone, a mixture of
xylitol and glucose and a mixture of xylitol and xylose. Xylitol metabolism was strictly respiratory and no ethanol was formed.
Simultaneous feeding of xylitol and glucose and xylitol and xylose to oxygen-limited xylitol-pregrown cells resulted in ethanol
formation. In vitro both pyruvate decarboxylase activity and alcohol dehydrogenase activity were present in cells metabolising
xylitol under oxygen-limited conditions; however, this did not result in ethanol formation. Glucose, xylose and xylitol utilisation,
respectively, were compared under anaerobic conditions with regard to growth rate, carbon source and oxygenation level during
pre-cultivation. Irrespective of pre-growth conditions, xylitol was not metabolised under anaerobic conditions, whereas ethanol
was formed from both xylose and glucose. Anaerobic xylose utilisation required induction of a xylose-utilising metabolic pathway
during pre-cultivation.
Received: 23 February 1999 / Received last revision: 20 July 1999 / Accepted: 1 August 1999 |
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