不同生境下川麦冬根围土壤丛枝菌根真菌多样性北大核心CSCD

采用高通量(Illumina Miseq)测序技术对栽培和野生2种生境下川麦冬根围的丛枝菌根(AM)真菌多样性和群落结构进行测定,并结合土壤理化因子进行相关性分析,以明确两种生境下川麦冬根围土壤AM真菌多样性和优势群落的分布特点,探讨AM真菌群落分布差异的驱动因子,为AM真菌应用于麦冬生产提供理论依据和技术支持。结果表明:(1)不同生境下川麦冬根围土壤中共鉴定出AM真菌3属10种,其中野生川麦冬根围土壤鉴定出的AM真菌3属7种,分别隶属于无梗囊霉属(Acaulospora)、多孢囊霉属(Diversispora)和球囊霉属(Glomus),而栽培环境下鉴定出AM真菌1属6种,隶属于球囊霉属。2个生境优势属均为球囊霉属。(2)不同生境下川麦冬根围AM真菌之间存在显著差异,野生生境下川麦冬根围土壤AM真菌多样性指数ACE和Shannon均显著高于人工栽培生境,而Simpson指数则相反。(3)相关性分析表明,AM真菌多样性指数及群落组成结构均与土壤理化因子存在相关性,其中全钾(TK)、全磷(TP)、全氮(TN)对AM真菌多样性指数和群落结构组成均存在显著影响。研究认为,不同生境下川麦冬根围AM真菌群落存在显著差异,球囊霉属为川麦冬互利共生的关键属,TK、TP、TN是不同生境川麦冬根围AM真菌群落差异的主要驱动因子。

Diversity of Arbuscular Mycorrhizal Fungi in the Rhizosphere of Ophiopogon japonicus in Different Habitats

In this study, Illumina Miseq sequencing technology was used to determine the diversity and community structure of arbuscular mycorrhizal (AM) fungi in the rhizosphere of Ophiopogon japonicus in both cultivated and wild habitats, and the correlation analysis was carried out in combination with soil physical and chemical factors, in order to clarify the distribution characteristics of AM fungi in the rhizosphere soil of O. japonicus in the two habitats and the distribution characteristics of dominant communities, explore the driving factors of the differences in the distribution of AM fungi communities, and provide theoretical basis and technical support for the application of AM fungi in the production of O. japonicus. The results showed that: (1) 10 species of AM fungi were identified from the rhizosphere soil of O. japonicus in different habitats. Among them, 7 species of AM fungi were identified from the rhizosphere soil of wild O. japonicus in 3 genera, belonging to the genera Acaulospora, Diversispora, and Glomus, respectively. 1 genus and 6 species of AM fungi were identified under the cultivation environment, belonging to the genus Glomus, and the dominant genus in both habitat were Glomus. (2) There were significant differences between the AM fungi in the rhizosphere of O. japonicus in different habitats. The AM fungi diversity index of the rhizosphere soil of O. japonicus in the wild habitat, ACE and Shannon, were both greater than those in the artificial habitat, while the Simpson index was the opposite. (3) Correlation analysis shows that AM fungal diversity index and community structure related to soil physical and chemical factors. Among them, total potassium (TK), total phosphorus (TP), and total nitrogen (TN) contributed to the differentiation of the AM fungal diversity index and community structure under different habitats. This study demonstrated that there are significant differences in the rhizosphere AM fungal community of O. japonicus in different habitats. Glomus is the key genus of O. japonicus mutually beneficial symbiosis. TK, TP and TN are the main driving factors for the differences in the rhizosphere AM fungal community of O. japonicus in different habitats.