A device to simplify the assay of ligand binding to cell surfaces |
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Authors: | Edward M Davis David D Tsay Max Schlamowitz Earl F Walborg Jr |
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Abstract: | A sensitive and reproducible technique for characterization of the binding of 125I-labeled protein ligands to cell surfaces is described. The physical separation of cell-bound and free ligand was accomplished by centrifugation-filtration using an assembly of plastic micro test tubes. This assembly allowed rapid and efficient separation of free and cell-bound ligands with minimal manipulation of the cells. |
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