|
|||||
|
|
Molecular cloning of the gene for dihydrofolate reductase from Lactobacillus casei |
|
| Authors: | R W Davies A M Gronenborn |
| Affiliation: | 1. Department of Biochemistry, University of Manchester, Institute of Science and Technology, P.O. Box 88, Manchester M60 1QD U.K.;7. National Institute for Medical Research, The Ridge-way, Mill Hill, London NW7 1AA U.K. |
| Abstract: | The Lactobacillus casei gene for dihydrofolate reductase has been cloned in Escherichia coli using the multicopy vector pBR322. A restriction map of the cloned DNA has been prepared. The cloned DNA directs the synthesis of L. casei dihydrofolate reductase in E. coli and confers trimethoprim and methotrexate resistance. |
| Keywords: | bp base pairs DHFR dihydrofolate reductase dYT medium 16 g Bacto tryptone 10 g yeast extract, 5 g NaCI per litre kb kilobase pairs Tet tetracycline TMP trimethoprim [] indicates plasmid-carrier state |
| 本文献已被 ScienceDirect 等数据库收录！ | |