Fertile transgenic plants obtained from tritordeum inflorescences by tissue electroporation |
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Authors: | G. Y. He P. A. Lazzeri M. E. Cannell |
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Affiliation: | (1) Biochemistry and Physiology Department, IACR-Rothamsted, Harpenden, Herts, AL5 2JQ, UK e-mail: guang.he@bbsrc.ac.uk Fax: +44-1582-763010, GB;(2) DuPont Wheat Transformation Laboratory, C/O IACR-Rothamsted, Harpenden, Herts, AL5 2JQ, UK, GB |
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Abstract: | Tissue electroporation was applied to a member of the Triticeae family, namely tritordeum (Hordeum chilense Roem.×Triticum turgidum L. Conv. durum), for the generation of fertile transgenic plants. Two transgenic plants were recovered following the treatment of 361 explants of immature inflorescences (although they were subsequently found to result from the same transformation event). The expression of both inserted marker genes (uidA and bar) was confirmed using standard assays, while transgene integration was confirmed using PCR and Southern hybridization analyses. Integration pattern, segregation ratio and the inheritance of transgene expression in T1 progeny were consistent for the presence of a single transgene locus containing five to ten plasmid insertions. Although this procedure has been applied to other cereal species, stable transformation of the Triticeae using tissue electroporation has not previously been reported. Received: 28 October 1999 / Revision received: 25 August 2000 / Accepted: 29 August 2000 |
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Keywords: | Triticeae Tritordeum Inflorescence Tissue electroporation Transgene inheritance |
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