| 新生BALB/c小鼠大脑皮质神经元细胞培养方法的建立 |
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| 引用本文： | 辛岗,苏芸,王革非,许燕璇,李康生.新生BALB/c小鼠大脑皮质神经元细胞培养方法的建立[J].生物技术通讯,2011,22(1):85-88. |
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| 作者姓名： | 辛岗 苏芸 王革非 许燕璇 李康生 |
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| 作者单位： | 汕头大学,医学院微生物与免疫学教研室,广东省免疫病理重点实验室,广东,汕头,515041 |
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| 基金项目： | 国家自然科学基金(30771988); 广东省医学科研基金(A2010392); 汕头市科技计划项目(汕府科[2009]70号) |
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| 摘 要： | 目的:经改良和优化,建立高纯度BALB/c小鼠大脑皮质神经元培养的方法.方法:采用L-多聚赖氨酸包被细胞培养板,取新生BALB/c小鼠(出生24 h内)大脑皮质组织,经0.25%胰酶消化后吹打成单个细胞,按1×106/孔接种于35 mm的六孔板中,用神经元细胞培养种植液培养6 h后换神经元细胞培养饲养液,培养40 h时...
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| 关 键 词： | 大脑皮质 神经元细胞 培养 BALB/c小鼠 |
Cultivation of Cerebral Cortex Neuronal Cells of Newborn BALB/c Mice |
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| XIN Gang,SU Yun,WANG Ge-Fei,XU Yan-Xuan,LI Kang-Sheng.Cultivation of Cerebral Cortex Neuronal Cells of Newborn BALB/c Mice[J].Letters in Biotechnology,2011,22(1):85-88. |
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| Authors: | XIN Gang SU Yun WANG Ge-Fei XU Yan-Xuan LI Kang-Sheng |
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| Institution: | XIN Gang,SU Yun,WANG Ge-Fei,XU Yan-Xuan,LI Kang-Sheng Department of Microbiology and Immunology,Shantou University Medical College,Key Immunopathology Laboratory of Guangdong Province,Shantou 515041,China |
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| Abstract: | Objective：To establish a method for cultivation of cerebral cortex neuronal cells of newborn BALB/c mice.Methods：The cortexes from newborn（less than 24 h） BALB/c mice were obtained and digested by 0.25% trypsin,and then dissociated into single cell suspension.About 1×10^6 cells were seeded onto each 35 mm dish which was coated by poly-L-lysine overnight previously.After cultivated in seeding medium for 6 h,the neuron cells were cultured in neurobasal medium containing B27,FBS,and glutamine.Cytosine arabinofuranoside was added to the cultures at a final concentration of 5 mg/mL on 40 h.Results：The neuron cells showed a typical morphorlogy at day 5.As indicated by indirect immunofluorescence using antibodies against neuron specific βⅢ tubulin,the purity of the neuronal cultures was 93%.Conlusion：The optimized method to culture neuron from BALB/c mice was established |
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| Keywords: | cerebral cortex neuronal cells culture BALB/c mice |
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