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| Myocardin协同维甲酸受体调节骨髓间充质干细胞向神经细胞分化 | |
| 引用本文： | 侯士芳,廖兴华,王楠,张同存,韩俊领. Myocardin协同维甲酸受体调节骨髓间充质干细胞向神经细胞分化[J]. 生物技术通讯, 2011, 22(1): 24-27. DOI: 10.3969/j.issn.1009-0002.2011.01.006 |
| 作者姓名： | 侯士芳 廖兴华 王楠 张同存 韩俊领 |
| 作者单位： | 1. 天津科技大学,生物工程学院分子药理实验室,天津,300222;协和干细胞基因工程有限公司,天津,300384 2. 天津科技大学,生物工程学院分子药理实验室,天津,300222 3. 协和干细胞基因工程有限公司,天津,300384 |
| 摘 要： | 目的:探讨Myocardin在维甲酸(RA)诱导骨髓间充质干细胞(BMSC)向神经细胞(NC)分化过程中的作用及与维甲酸X受体α(RXRa)的相互作用.方法:免疫细胞荧光检测RA诱导BMSC向NC分化;RT-PCR检测Myocardin及RXRa在RA诱导BMSC分化为神经细胞过程中的表达;萤光素酶活性实验分析Myoc... |
| 关 键 词： | Myocardin 维甲酸 维甲酸X受体α 骨髓间充质干细胞 神经细胞 |
Cooperative Effect of Rretinoid X Receptor Alpha and Myocardin in Regulation of Neural Cell Differentiation |
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| HOU Shi-Fang,LIAO Xing-Hua,WANG Nan,ZHANG Tong-Cun,HAN Jun-Ling. Cooperative Effect of Rretinoid X Receptor Alpha and Myocardin in Regulation of Neural Cell Differentiation[J]. Letters in Biotechnology, 2011, 22(1): 24-27. DOI: 10.3969/j.issn.1009-0002.2011.01.006 | |
| Authors: | HOU Shi-Fang LIAO Xing-Hua WANG Nan ZHANG Tong-Cun HAN Jun-Ling |
| Affiliation: | HOU Shi-Fang1,2,LIAO Xing-Hua1,WANG Nan1,ZHANG Tong-Cun1,HAN Jun-Ling2 1.Laboratory for Molecular Pharmacology,College of Bioengineering,Tianjin University of Science & Technology,Tianjin 300222,2.Union Stemcell & Gene Engineering Co.Ltd,Tianjin 300384,China |
| Abstract: | Objective:To discover the role of myocardin in the differentiation of bone marrow stromal cells(BMSC) into neural cells under induction by retinoic acid(RA),and investigate the cooperation with retinoid X receptor alpha(RXRa).Methods:Expression of NSE and MAP-2 in BMSC treated with RA were tested by immunohistochemcal assay;mRNA expression level of myocardin and RXRa in differentiation of BMSC into neural cells were tested with RT-PCR.The luciferase assay was used to investigated the effection of RXRa and m... |
| Keywords: | myocardin retinoic acid retinoid X receptor alpha bone marrow stromal cells neural cells |
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