| 伤寒沙门菌和甲型副伤寒沙门菌分离株的外膜蛋白谱比较 |
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| 引用本文： | 张力,王瑞白,闫梅英,肖迪,阚飙. 伤寒沙门菌和甲型副伤寒沙门菌分离株的外膜蛋白谱比较[J]. 生物技术通讯, 2011, 22(2): 173-177. DOI: 10.3969/j.issn.1009-0002.2011.02.006 |
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| 作者姓名： | 张力 王瑞白 闫梅英 肖迪 阚飙 |
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| 作者单位： | 中国疾病预防控制中心,传染病预防控制所,传染病预防控制国家重点实验室,北京,102206 |
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| 基金项目： | 国家科技重大专项(2008ZX10004-002) |
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| 摘 要： | 目的:比较伤寒沙门菌和甲型副伤寒沙门菌流行菌株的外膜蛋白谱差异。方法:运用二维蛋白电泳方法,对我国伤寒沙门菌株XJ90和甲型副伤寒沙门菌株JX2005-92在实验室通用营养条件下培养提取的外膜蛋白进行分离,比对其差异,对差异蛋白点进行质谱鉴定,对鉴定蛋白点的基因序列也进行比较。结果:菌株XJ90中发现20个特异蛋白点,质谱鉴定出16个;菌株JX2005-92中发现29个特异蛋白点,鉴定出18个。在这些蛋白中,OmpA是数目最多的同种差异蛋白。这些差异蛋白点中的大部分编码基因在2种细菌中序列高度相似或相同。结论:伤寒沙门菌和甲型副伤寒沙门菌基因序列高度相似的外膜蛋白具有不同的修饰形式,提示其不同遗传背景在相同的环境条件下表现出精细的功能差异。
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| 关 键 词： | 外膜蛋白 二维电泳 伤寒沙门菌 甲型副伤寒沙门菌 |
Proteomic Comparison of Outer Membrane Proteins of Salmonella Typhi and Paratyphi A Isolates |
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| ZHANG Li,WANG Rui-Bai,YAN Mei-Ying,XIAO Di,KAN Biao. Proteomic Comparison of Outer Membrane Proteins of Salmonella Typhi and Paratyphi A Isolates[J]. Letters in Biotechnology, 2011, 22(2): 173-177. DOI: 10.3969/j.issn.1009-0002.2011.02.006 |
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| Authors: | ZHANG Li WANG Rui-Bai YAN Mei-Ying XIAO Di KAN Biao |
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| Affiliation: | ZHANG Li,WANG Rui-Bai,YAN Mei-Ying,XIAO Di,KAN Biao State Key Laboratory for Infections Disease Prevention and Control,National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China |
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| Abstract: | Objective:To reveal the outer membrane protein(OMP) profile difference between Salmonella Typhi and Paratyphi A isolates.Methods:Two-dimensional gel electrophoresis was used to separate the OMP extracts from the Salmonella Typhi strain XJ90 and Salmonella Paratyphi A strain JX2005-92,which were cultures under the common media in laboratory.The OMP profiles of these two strains were compared and the different protein spots were identified with mass spectrometry.Nucleic acid sequences of genes of the identifi... |
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| Keywords: | outer membrane protein Salmonella Typhi Salmonella Paratyphi A 2-D gel electrophoresis |
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