| 水稻幼苗特异应答H2O2的miRNA169b启动子表达载体的构建 |
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| 引用本文： | 徐文,王正明,刘进元,刘卫群. 水稻幼苗特异应答H2O2的miRNA169b启动子表达载体的构建[J]. 生物技术通讯, 2011, 22(2): 234-237. DOI: 10.3969/j.issn.1009-0002.2011.02.020 |
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| 作者姓名： | 徐文 王正明 刘进元 刘卫群 |
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| 作者单位： | 1. 河南农业大学,生命科学学院,河南,郑州,450002
2. 清华大学,生命科学学院,北京,100084 |
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| 基金项目： | 国家自然科学基金(30971704) |
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| 摘 要： | 目的:研究microRNA169b(miRNA169b)启动子不同长度片段的活性及自身调控。方法:用生物信息学方法分析水稻中miRNA169b前体上游1500 bp序列,并从水稻幼苗基因组DNA中扩增miRNA169b前体上游500、1000和1500 bp启动子片段,分别将3个片段克隆至萤光素酶报告基因载体p5XGal4-LUC上。结果:构建了miRNA169b启动子重组载体,序列分析表明与预期结果一致。结论:miRNA169b启动子调控的萤光素酶报告基因表达载体的构建,为研究水稻中miRNA基因自身的转录奠定了基础。
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| 关 键 词： | 水稻 微小RNA 启动子 H2O2 载体构建 |
Construction of Expression Vectors Driven by H2O2-Responsive miRNA169b Promoter in Rice Seedlings |
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| XU Wen,WANG Zheng-Ming,LIU Jin-Yuan,LIU Wei-Qun. Construction of Expression Vectors Driven by H2O2-Responsive miRNA169b Promoter in Rice Seedlings[J]. Letters in Biotechnology, 2011, 22(2): 234-237. DOI: 10.3969/j.issn.1009-0002.2011.02.020 |
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| Authors: | XU Wen WANG Zheng-Ming LIU Jin-Yuan LIU Wei-Qun |
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| Affiliation: | XU Wen1,WANG Zheng-Ming2,LIU Jin-Yuan2,LIU Wei-Qun1 1.College of Life Science,Henan Agricultural University,Zhengzhou 450002,2.Department of Biological Sciences and Biotechnology,Tsinghua University,Beijing 100084,China |
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| Abstract: | Objective:To research activity and self-regulation of different segments of miRNA169b promoter in rice.Methods:The 1500,1000 and 500 bp DNA segment of rice miRNA169b promoter were analyzed with bioinformatics and amplified by PCR from rice seedlings and cloned to p5XGal4-LUC vectors,then the positive clones were confirmed by restriction enzymes and sequencing.Results:The targeted segments were cloned to Firefly luciferase report vectors p5XGal4-LUC.Conclusion:These results may make an important basis for fu... |
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| Keywords: | Oryza sativa microRNA promoter construction of vectors |
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