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| PES1慢病毒表达载体的构建及其对乳腺癌ZR75-30细胞生长的影响 | |
| 引用本文： | 程龙,韩永健,蒋凯,曹佳,张浩,徐小洁,朱建华,杨智洪,叶棋浓. PES1慢病毒表达载体的构建及其对乳腺癌ZR75-30细胞生长的影响[J]. 生物技术通讯, 2011, 22(2): 196-198,219. DOI: 10.3969/j.issn.1009-0002.2011.02.011 |
| 作者姓名： | 程龙 韩永健 蒋凯 曹佳 张浩 徐小洁 朱建华 杨智洪 叶棋浓 |
| 作者单位： | 1. 军事医学科学院,生物工程研究所,北京,100850 2. 曲阜师范大学,生命科学学院,山东,曲阜,273100 3. 解放军总医院,第一附属医院肿瘤科,北京,100037 |
| 基金项目： | 国家自然科学基金(30800205,30872939) |
| 摘 要： | 目的:利用慢病毒载体表达PES1基因,研究其对乳腺癌ZR75-30细胞生长的影响.方法:以乳腺文库为模板,PCR扩增PES1基因,克隆到pCDH载体,构建成pCDH-PES1,将其与包装载体共转293T细胞,包装成Lenti-PES1慢病毒载体并测定病毒滴度,感染乳腺癌ZR75-30细胞,Western印迹鉴定病毒载体... |
| 关 键 词： | PES1 慢病毒载体 乳腺癌 细胞生长 |
Construction of a Lentiviral Vector for Overexpression of PES1 and its Effect on the Growth of ZR75-30 Breast Cancer Cells |
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| CHENG Long,HAN Yong-Jian,JIANG Kai,CAO Jia,ZHANG Hao,XU Xiao-Jie,ZHU Jian-hua,YANG Zhi-Hong,YE Qi-Nong. Construction of a Lentiviral Vector for Overexpression of PES1 and its Effect on the Growth of ZR75-30 Breast Cancer Cells[J]. Letters in Biotechnology, 2011, 22(2): 196-198,219. DOI: 10.3969/j.issn.1009-0002.2011.02.011 | |
| Authors: | CHENG Long HAN Yong-Jian JIANG Kai CAO Jia ZHANG Hao XU Xiao-Jie ZHU Jian-hua YANG Zhi-Hong YE Qi-Nong |
| Affiliation: | CHENG Long1,HAN Yong-Jian1,JIANG Kai1,CAO Jia2,ZHANG Hao1,XU Xiao-Jie1,ZHU Jian-hua3,YANG Zhi-Hong1,YE Qi-Nong1 1.Beijing Institute of Biotechnology,Beijing 100850,2.College of Life Sciences,Qufu Normal University,Qufu 273100,3.Department of Oncology,First Affiliated Hospital,China PLA General Hospital,Beijing 100037,China |
| Abstract: | Objective:To construct and identify a lentiviral vector for overexpression of PES1,and to study its effect on the growth of the breast cancer cell line ZR75-30.Methods:PES1 gene was amplified from breast library using PCR and cloned into the pCDH vector.The resulting plasmid pCDH-PES1 and pPack Packaging Plasmid Mix were co-transfected into 293T cells to obtain packaged lentivirus particles.The titers of virus were tested.Western blot was performed to identify the lentivirus-mediated expression of PES1 in Z... |
| Keywords: | PES1 lentivirus vector breast cancer cell growth |
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