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| 微环DNA研究进展 | |
| 引用本文： | 胡春生,张通,张庆林. 微环DNA研究进展[J]. 生物技术通讯, 2011, 22(1): 104-107,112. DOI: 10.3969/j.issn.1009-0002.2011.01.024 |
| 作者姓名： | 胡春生 张通 张庆林 |
| 作者单位： | 1. 内蒙古工业大学,化工学院,内蒙古,呼和浩特,010051;军事医学科学院,放射与辐射医学研究所,北京,100850 2. 内蒙古工业大学,化工学院,内蒙古,呼和浩特,010051 3. 军事医学科学院,放射与辐射医学研究所,北京,100850 |
| 摘 要： | 质粒载体在基因治疗中占据重要地位.传统质粒DNA在真核生物中可能会引起严重的炎症反应,未甲基化的CpG序列可能抑制基因的表达,最好的解决办法是在生产质粒载体过程中将细菌调控序列整体消除.微环DNA是一种新颖的小环超螺旋表达框,它是传统质粒在大肠杆菌体内通过位点特异性重组得到的.微环DNA缺乏抗性标记基因、复制原点等细菌... |
| 关 键 词： | 微环DNA 纯化 重组酶 进展 |
Research Progress of Minicircle DNA |
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| HU Chun-Shen,ZHANG Tong,ZHANG Qing-Lin. Research Progress of Minicircle DNA[J]. Letters in Biotechnology, 2011, 22(1): 104-107,112. DOI: 10.3969/j.issn.1009-0002.2011.01.024 | |
| Authors: | HU Chun-Shen ZHANG Tong ZHANG Qing-Lin |
| Affiliation: | HU Chun-Sheng1,2,ZHANG Tong1,ZHANG Qing-Lin2 1.Chemical Engineering College of Inner Mongolia University of Technology,Hohhot 010051,2.Beijing Institute of Radiation Medicine,Beijing 100850,China |
| Abstract: | Plasmid DNA play an important role in gene therapy.Plasmid DNA used for non-viral gene delivery may cause unacceptable inflammatory responses in eukaryotes.Unmethylated CpG is likely to inhibit gene expression.A good solution is to entirely eliminate the bacterial control regions from gene delivery vectors during the process of plasmid production.Minicircle DNA is a novel supercoiled minimal expression cassette containing the eukaryotic gene,derived from conventional plasmid DNA by site-specific recombinati... |
| Keywords: | minicircle DNA purification recombinase progress |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |