| 准噶尔小胸鳖甲抗冻蛋白MpAFP698在真核细胞中的表达与鉴定 |
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| 引用本文： | 姜敏,于继云,李晨晨,马纪.准噶尔小胸鳖甲抗冻蛋白MpAFP698在真核细胞中的表达与鉴定[J].生物技术通讯,2011,22(2):154-157. |
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| 作者姓名： | 姜敏 于继云 李晨晨 马纪 |
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| 作者单位： | 1. 新疆大学,生命科学与技术学院,新疆生物资源基因工程重点实验室,新疆,乌鲁木齐,830046
2. 军事医学科学院,基础医学研究所,北京,100850 |
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| 基金项目： | 国家自然科学基金(30760056); 新疆生物资源基因工程重点实验室开放课题(XJDX0201-200804) |
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| 摘 要： | 目的:构建真核表达质粒pEGFP-N1-mpafp698,转染人胚肾293T细胞,并表达准噶尔小胸鳖甲抗冻蛋白MpAFP698.方法:PCR扩增出mpafp698序列,将其克隆入本室保存的真核表达载体pEGFP-N1中,转染293T细胞;利用RT-PCR、流式细胞仪、免疫荧光、Western 印迹检测蛋白表达.结果:构...
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| 关 键 词： | 准噶尔小胸鳖甲 抗冻蛋白 细胞表达 |
Expression and Characterization of Microdera punctipennis dzhungarica Antifreeze Protein MpAFP698 in Eukaryotic Cell |
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| JIANG Min,YU Ji-Yun,LI Chen-Chen,MA Ji.Expression and Characterization of Microdera punctipennis dzhungarica Antifreeze Protein MpAFP698 in Eukaryotic Cell[J].Letters in Biotechnology,2011,22(2):154-157. |
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| Authors: | JIANG Min YU Ji-Yun LI Chen-Chen MA Ji |
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| Institution: | JIANG Min2,YU Ji-Yun1,LI Chen-Chen2,MA Ji2 1.Institute of Basic Medical Sciences,Academy of Military Medical Sciences,Beijing 100850,2.Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,College of Life Science and Technology,Xinjiang University,Urumqi 830046,China |
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| Abstract: | Objective:To construction eukaryotic expression vector pEGFP-N1-mpafp698 and express Microdera punctipennis dzhungarica antifreeze protein MpAFP698 in eukaryotic cell 293T by transfection.Methods:The segments of mpafp698 was obtained by the method of PCR and was inserted into a eukaryotic expression plasmid pEGFP-N1.The recombinant expression plasmid was tansfected into eukaryotic cell 293T,and the expression was detected by RT-PCR,FACS,Western blot and IMF.Results:The recombinant plasmid pEGFP-N1-mpafp698 ... |
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| Keywords: | Microdera punctipennis dzhungarica antifreeze protein cell expression |
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