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| 慢性髓细胞性白血病病人骨髓单个核细胞中差异表达基因的筛选 | |
| 引用本文： | 王蜀燕,孙朝晖,危敏,马文丽.慢性髓细胞性白血病病人骨髓单个核细胞中差异表达基因的筛选[J].生物技术通讯,2011,22(2):203-207. |
| 作者姓名： | 王蜀燕 孙朝晖 危敏 马文丽 |
| 作者单位： | 1. 广州军区,广州总医院医务部,东,广州,510010 2. 广州军区,广州总医院检验科,广东,广州,510010 3. 南方医科大学,基因工程研究所,广东,广州,510515 |
| 基金项目： | 广东省自然科学基金(7300155) |
| 摘 要： | 目的:筛选慢性髓细胞性白血病(CML)病人骨髓单个核细胞与正常人的差异表达基因,探讨CML的发病机制.方法:提取正常人和CML病人单个核细胞的RNA,逆转录成cDNA并用地高辛标记,应用全基因组表达谱基因芯片对差异表达基因进行研究,采用Jubilant病理/疾病分类法对CML相关差异表达基因进行分析.结果:共筛选出CM... |
| 关 键 词： | 慢性髓细胞性白血病 寡核苷酸微阵列 基因表达谱 |
Screen Differential Expressed Genes in Marrow Mononuclearcell of Chronic Myelogenous Leukemia Patient |
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| WANG Shu-Yan,SUN Zhao-Hui,WEI Min,MA Wen-Li.Screen Differential Expressed Genes in Marrow Mononuclearcell of Chronic Myelogenous Leukemia Patient[J].Letters in Biotechnology,2011,22(2):203-207. | |
| Authors: | WANG Shu-Yan SUN Zhao-Hui WEI Min MA Wen-Li |
| Institution: | WANG Shu-Yan,SUN Zhao-Hui,WEI Min,MA Wen-Li Medical Department,General Hospital of Guangzhou Command,Guangzhou 510010,China |
| Abstract: | Objective:To screen differential expressed genes in marrow mononuclearcell of chronic myelogenous leukemia(CML) patient in order to develop the mechanism of CML.Methods:RNA were extracted in marrow mononuclearcell of both normal person and CML patient marked with digoxin.Differential expressed genes were analyzed by ABI 1700 bioarray and sorted by Jubilant physiology/disease categories.Results:A total of 6706 genes had significant differential expressed 43 genes relating to CML which have 13 genes up-regula... |
| Keywords: | chronic myelogenous leukemia oligonucleotide microarray gene expressed spectrum |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |