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Cloning and Expression Analysis of a Putative ABC Transporter Gene BgABC1 from the Biotrophic Pathogenic Fungus Blumeria graminis f. sp. tritici
Authors:W. Hu    L. Yan    Z. Ma
Affiliation:Authors' addresses: Institute of Biotechnology, Zhejiang University, 268 Kaixuan Road, Hangzhou 310029, China;;Department of Bioassay, National Southern Center for Research and Development of Pesticide, Hangzhou 310023, China (correspondence to Z. Ma. E-mail: )
Abstract:A putative ATP‐binding cassette (ABC) transporter gene (BgABC1) was isolated from the biotrophic pathogenic fungus Blumeria graminis f. sp. tritici (Bgt). Analysis of the deduced amino acid sequence of BgABC1 showed that the BgABC1 protein had a conserved nucleotide‐binding fold in the N‐terminus, and six transmembrane domains (TMDs) in the C‐terminus. Analysis of the BgABC1 expression using real‐time polymerase chain reaction showed that expression of the gene was increased significantly when the fungus was growing in wheat seedlings treated with 14α‐demethylase‐inhibiting fungicide, triadimefon. The results indicated that the BgABC1 was involved in the protection of the fungus against fungicide toxicity.
Keywords:ATP-binding cassette transporter    Blumeria graminis f. sp. tritici    14α-demethylase-inhibiting fungicide    real-time polymerase chain reaction
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