Two unisexual artificial polyploid clones constructed by genome addition of common carp (<Emphasis Type="Italic">Cyprinus carp</Emphasis>) and crucian carp (<Emphasis Type="Italic">Carassius auratus</Emphasis>) |
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Authors: | Email author" target="_blank">Qingjiang?WuEmail author Yuzhen?Ye Xinhong?Dong |
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Institution: | State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China |
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Abstract: | A polyploid hybrid fish with natural gynogenesis can prevent segregation and maintain their hybrid vigor in their progenies.
Supposing the reproduction mode of induced polyploid fish being natural gynogenesis, allopolyploid hybrid between common carp
and crucian carp into allopolyploid was performed. The purpose of this paper is to describe a lineage from sexual diploid
carp transforming into allotriploid and allotetraploid unisexual clones by genome addition. The diploid hybrid between common
carp and crucian carp reproduces an unreduced nucleus consisting of two parental genomes. This unreduced female pronucleus
will fuse with male pronucleus and form allotriploid zygote after penetration of related species sperms. Allotriploid embryos
grow normally, and part of female allotriploid can produce unreduced mature ova with three genomes. Mature ova of most allotriploid
females are provided with natural gynogenetic trait and their nuclei do not fuse with any entrance sperm. All female offspring
are produced by gynogenesis of allotriploid egg under activation of penetrating sperms. These offspring maintain morphological
traits of their allotriploid maternal and form an allotetraploid unisexual clone by gynogenetic reproduction mode. However,
female nuclei of rare allotriploid female can fuse with penetrating male pronuclei and result in the appearance of allotetraploid
individuals by means of genome addition. All allotetraploid females can reproduce unreduced mature eggs containing four genomes.
Therefore, mature eggs of allotetraploid maintain gynogenetic trait and allotetraploid unisexual clone is produced under activation
of related species sperms. |
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Keywords: | carp unreduced genome addition allopolyploid gynogenesis clone |
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