The biosynthesis of biotin in growing yeast cells: The formation of biotin from an early intermediate |
| |
Authors: | Eisenberg M A |
| |
Affiliation: | Department of Biochemistry, College of Physicians and Surgeons, Columbia University, New York, N.Y., U.S.A. |
| |
Abstract: | 1. Yeast cells grown in the presence of an unknown radioactive biotin vitamer produced by Penicillium chrysogenum incorporated the vitamer into the newly synthesized biotin. 2. The biotin was isolated as the avidin–biotin complex and after hydrolysis the biological activity and radioactivity were shown to be coincidental. 3. The specific activity of the biotin was identical with that of the pimelic acid used in a previous investigation to label the unknown vitamer. 4. The role of the unknown biotin vitamer as an intermediate in biotin biosynthesis is discussed. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|