The use of EDTA or polymyxin with lysozyme for the recovery of intracellular products fromEscherichia. coli |
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Authors: | C. R. Dean O. P. Ward |
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Affiliation: | (1) Department of Biology, University of Waterloo, N2L 3G1 Waterloo, Ontario, Canada |
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Abstract: | Summary Escherichia coli cells taken from exponential and late stationary (or decline) phases of culture were very susceptible to lysis by EDTA/lysozyme. Log phase cells were most susceptible to lysis by polymyxin/lysozyme. Treatment ofE. coli with EDTA and lysozyme compared favourably with sonication as a method for release of intracellular protein. Concentration ranges for optimal lysis were 100–800 μg/ml for EDTA and 25–50 μg/ml for lysozyme. |
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