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A rapid fluorometric method for the estimation of DNA in cultured cells |
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| Authors: | Bradley G Erwin Christa M Stoscheck James R Florini |
| Institution: | 1. Biology Department, Syracuse University, Syracuse, New York 13210, USA;1. Department of Physiology, Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA;2. Department of Biochemistry, Vanderbilt University, Nashville, Tennessee 37232 USA |
| Abstract: | We present here a procedure for the rapid measurement of both DNA and protein from the same aliquot of cell lysate. DNA estimates obtained by this method were compared to replicate determinations using the method of Kissane and Robins (1). The optimal range for the estimation of DNA (1 to 20 μg) is well suited for use with portions of extracts from individual cell cultures; the remainder of the extract remains available for enzyme assays or other parallel determinations. |
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