Initiation of chromosomal DNA synthesis in vitro |
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| Authors: | Steven J. Projan and James A. Wechsler |
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| Affiliation: | (1) Department of Biology, University of Utah, 84112 Salt Lake City, Utah;(2) Department of Biological Sciences, Columbia University, 10027 New York, New York, USA;(3) Present address: The Public Health Research Institute of the City of New York, Inc., 455 First Avenue, 10016 New York, New York, USA |
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| Abstract: | Summary An in vitro complementation assay for initiation of chromosomal DNA replication is described. The initiation reaction is dependent upon extract from either of two hybrid-plasmid containing strains. Each hybrid plasmid carries a suppressor of dnaA-ts mutations. The in vitro DNA synthesis is heavily biased toward the origin region, and the origin of replication (oriC) is replicated as determined by DNA-DNA hybridizations. |
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