Isolation of highly purified cyclodextrin glucanotransferase from Bacillus sp. 1070 |
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Authors: | Volkova D A Lopatin S A Gracheva I M Varlamov V P |
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Institution: | Center of Bioengineering, RAS, Moscow, 117312. |
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Abstract: | Two chromatographic processes for purification of cyclodextringlucanotransferase (CGTase) from Bacillus sp. 1070 was carried out. The enzyme has been purified into 9.5 times on Butyl-Toyopearl and followed immobilized metal ion chromatography on Cu(II)-Iminodiacetic (IDA)-agarose. By the application of second purification scheme (chromatography on Butyl-Toyopearl and DEAE-Sephacel) the specific activity of CGTase has folded into 13.5 times. The purity of enzyme was shown to be approximately 90% by SDS-electrophoreses data. It was shown that isolated enzyme has two isoelectric points estimated as 5.1 and 5.3. |
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