Functional integration of serial dilution and capillary electrophoresis on a PDMS microchip |
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Authors: | Email author" target="_blank">Jun?Keun?ChangEmail author Yun?Seok?Heo Hyunwoo?Bang Keunchang?Cho Seok?Chung Chanil?Chung Dong?Chul?Han |
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Institution: | (1) School of Electrical Eng. & Computer Science, Seoul National University, 151-742 Seoul, Korea;(2) Digital Bio Technology, Co., Institute of Advanced Machinery and Design, Seoul National University, 151-742 Seoul, Korea;(3) School of Mechanical and Aerospace Engineering, Seoul National University, 151-742 Seoul, Korea |
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Abstract: | For the quantitative analysis of an unknown sample a calibration curve should be obtained, as analytical instruments give
relative, rather than absolute measurements. Therefore, researchers should make standard samples with various known concentrations,
measure each standard and the unknown sample, and then determine the concentration of the unknown by comparing the measured
value to those of the standards. These procedures are tedious and time-consuming. Therefore, we developed a polymer based
microfluidic device from polydimethylsiloxane, which integrates serial dilution and capillary electrophoresis functions in
a single device. The integrated microchip can provide a one-step analytical tool, and thus replace the complex experimental
procedures. Two plastic syringes, one containing a buffer solution and the other a standard solution, were connected to two
inlet holes on a microchip, and pushed by a hydrodynamic force. The standard sample is serially diluted to various concentrations
through the microfluidic networks. The diluted samples are sequentially introduced through microchannels by electro-osmotic
force, and their laser-induced fluorescence signals measured by capillary electrophoresis. We demonstrate the integrated microchip
performance by measuring the fluorescence signals of fluorescein at various concentrations. The calibration curve obtained
from the electropherograms showed the expected linearity. |
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Keywords: | serial dilution capillary electrophoresis microchip polydimethylsiloxane (PDMS) labon-a-chip |
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