| HIV-1衣壳蛋白在转基因枸杞中表达的免疫组织化学定位 |
| |
| 引用本文： | 刘丹如,宋长征,张更林,杜国利.HIV-1衣壳蛋白在转基因枸杞中表达的免疫组织化学定位[J].生物技术通讯,2006,17(5):719-721. |
| |
| 作者姓名： | 刘丹如 宋长征 张更林 杜国利 |
| |
| 作者单位： | 卫生部生物技术药物重点实验室,山东省医药生物技术研究中心,山东省医学科学院,山东,济南,250062 |
| |
| 基金项目： | 国家自然科学基金项目(30271215,30471621) |
| |
| 摘 要： | 目的:研究转基因植物中重组蛋白的细胞定位,有助于进一步了解转基因枸杞中HIV-1衣壳(CA)蛋白融合蛋白的分泌表达途径。方法:利用含有MA4-CA融合基因的农杆菌转化枸杞,转化植株获得再生。采用免疫组织化学方法对转基因枸杞表达的CA融合蛋白进行初步定位。结果:免疫组织化学定位表明,在转基因枸杞愈伤组织中,HIV-1CA主要在细胞浆、细胞壁和细胞间隙中表达。结论:免疫组织化学结果初步证明了CA融合蛋白在转基因枸杞中的表达分布。
|
| 关 键 词： | 人类免疫缺陷病毒 衣壳蛋白 免疫组织化学 转基因植物 枸杞 |
| 文章编号： | 1009-0002(2006)05-0719-03 |
| 收稿时间： | 12 26 2005 12:00AM |
| 修稿时间： | 2005年12月26 |
Immunolocalization of Recombinant HIV-1 Capsid Protein Secreted from Transgenic Lycium barbarum L. |
| |
| LIU Dan-ru,SONG Chang-zheng,ZHANG Geng-lin,DU Guo-li.Immunolocalization of Recombinant HIV-1 Capsid Protein Secreted from Transgenic Lycium barbarum L.[J].Letters in Biotechnology,2006,17(5):719-721. |
| |
| Authors: | LIU Dan-ru SONG Chang-zheng ZHANG Geng-lin DU Guo-li |
| |
| Institution: | Key Laboratory for Biotech-Drugs of Ministry of Health, Shandong Medicinal Biotechnology Center, Shandong Academy of Medical Sciences, Jinan 250062, China |
| |
| Abstract: | Objective: To explore the secretory pathway of recombinant HIV-1 capsid(CA) protein produced using root secretion method(rhizosecretion) in the transgenic Lycium barbarum L. Methods: The MA4-CA fusion gene was introduced into the genome of L.barbarum by using Agrobacterium tumefaciens-mediated gene transfer. The transformed internodes were regenerated. The recombinant HIV-1 CA was primarily immunolocalized by immunohistochemistry. Results: Immunohistochemistry of the transgenic callus showed that CA protein was mainly expressed in the cytoplasm, cell wall and intercellular space, little in nucleus. Conclusion: The results initially indicated that recombinant capsid protein was secreted from transgenic L.barbarum L. |
| |
| Keywords: | human immunodeficiency virus capsid protein immunohistochemistry transgenic plant Lycium barbarum L |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
