3-Hydroxybenzoate 4-hydroxylase from Pseudomonas testosteroni |
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Authors: | J L Michalover D W Ribbons H Hughes |
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Institution: | 1. Department of Biochemistry University of Miami School of Medicine Miami, Florida, 33152, U.S.A.;2. Howard Hughes Medical Institute Miami, Florida, 33152, U.S.A. |
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Abstract: | 3-Hydroxybenzoate 4-hydroxylase has been purified to homogeneity from extracts pf . It is a flavoprotein (FAD) which catalyzes the transformation of 3 -hydroxybenzoate to protocatechuate with equimolar consumption of NADPH and O2. NADH is a poor substitute for NADPH. Several analogues of 3-hydroxybenzoate substituted in the 2,4,5 and 6 positions, act as effectors and substrates for NADPH oxidation but with varying efficiencies of hydroxylation. 2,3-, 2,5-, 3,5-dihydroxybenzoates, 3-hydroxyanthranilate, 2-fluoro-5-hydroxybenzoate and 4-fluoro-3-hydroxybenzoate are competent substrates. |
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