Making the Most of Fusion Tags Technology in Structural Characterization of Membrane Proteins |
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| Authors: | Hao Xie Xiao-Ming Guo Hong Chen |
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| Institution: | (1) Department of Biological Science and Biotechnology, Institute of Science, Wuhan University of Technology, 122 Luoshi Road, Wuhan, 430070, People’s Republic of China;(2) School of Materials Science and Engineering, Wuhan University of Technology, 122 Luoshi Road, Wuhan, 430070, People’s Republic of China |
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| Abstract: | Membrane proteins can be investigated at various structural levels, including the topological structure, the high-resolution
three-dimensional structure, and the organization and assembly of membrane protein complexes. Gene fusion technology makes
it possible to insert a polynucleotide encoding a protein or polypeptide tag into the gene encoding a membrane protein of
interest. Resultant recombinant proteins may possess the functions of the original membrane proteins, together with the biochemical
properties of the imported fusion tag, greatly enhancing functional and structural studies of membrane proteins. In this article,
the latest literature is reviewed in relation to types, applications, strategies, and approaches to fusion tag technology
for structural investigations of membrane proteins. |
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| Keywords: | Membrane proteins Fusion tags Topological structure 3D structure Membrane protein complexes |
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