Cloning of sucrase operon with mini-mu and plasmid-mediated metabolism of sucrose |
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Authors: | J Grones M Mačor V Bilská |
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Institution: | (1) Department of Molecular Biology, Faculty of Science, Comenius University, 842 15 Bratislava, Slovak Republic |
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Abstract: | Thein vivo cloning system based on mini-Mu derivatives was used for cloning the sucrase operon. We constructed several recombinant plasmids
pJT21, pMM2324, pMM2325 with a complete sucrase operon. Two strains ofEscherichia coli containing this plasmid replicon were able to grow on different concentrations of sucrose. The stability of recombinant plasmids
was determined after cultivation under nonselective conditions. The stability after 5-d cultivation was higher than 75%. |
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