Simultaneous measurement of superoxide generation and intracellular calcium ion of neutrophil-like culture cells. |
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Authors: | Hiroshi Satozono Kimiko Kazumura Sigetoshi Okazaki Mitsuo Hiramatsu |
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Affiliation: | Hamamatsu Photonics K.K., 5000 Hirakuchi, Hamamatsu-City 434-8601, Japan. satozono@crl.hpk.co.jp |
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Abstract: | We have developed a novel method for simultaneously measuring fluorescence and chemiluminescence. The generation of superoxide anion and the intracellular Ca(2+) ion concentration of neutrophil-like cells stimulated by agonists were measured in real time by our method. Our results were in agreement with the intracellular signalling in the neutrophils. We also found that the presence of Zn(2+) ion inhibited both the generation of superoxide anion and the influx of Ca(2+) ions. |
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Keywords: | superoxide calcium neutrophil chemiluminescence fluorescence |
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