Microchamber arrays for the identification of individual cells exposed to an X-ray microbeam |
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Authors: | Takahiro Kuchimaru Fuminobu Sato Yusuke Aoi Tomohisa Fujita Toshiji Ikeda Kikuo Shimizu Yushi Kato Toshiyuki Iida |
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Affiliation: | (1) Division of Electric, Electronic and Information Engineering, Graduate School of Engineering, Osaka University, Suita Osaka, 567-0871, Japan;(2) Institute of Scientific and Industrial Research, Osaka University, Suita, Osaka, Japan;(3) Radio Isotope Research Center, Osaka University, Suita, Osaka, Japan |
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Abstract: | To identify individual cells exposed to a X-ray microbeam in a cell population, we developed a biocompatible microchamber-array chip using UV lithography of photopolymer SU-8. The center-to-center distance between microchambers is 50 μm including a wall of 15 μm height. Using the microchamber-array chip, we performed tracking of individual exposed cells. Sample cells loaded in a microchamber array were selectively irradiated with the X-ray microbeam under microscopic observation. All the irradiated cells were indexed by the array arrangement of the microchambers. For about 24 h of post-irradiation incubation, the irradiated cells were identified successfully by time-lapse observation. In addition, the induction of radiation effects was observed in identified cells using immunofluorescence. |
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