利用有序差异显示技术克隆受稻瘟病菌诱导表达的水稻cDNA的研究

利用有序差异显示技术(ODD)分析受稻瘟病菌诱导表达的水稻基因，通过反式RNA印迹进行辅助筛选，获得了37个在接种稻瘟病菌后表达量增强的水稻cDNA克隆.采用RNA印迹对其中5个克隆在接种稻瘟病菌后的表达分析表明，这些克隆在抗病以及感病的水稻株系中都具有诱导表达的特点.根据序列同源性分析，与这些克隆序列相应的同源基因可能涉及抑制病原菌生长、清除真菌毒素、传递抗病信号以及调节宿主生理状态等几方面的功能.

Application of Ordered Differential Display to Isolate Rice cDNAs Induced by Magnaporthe grisea

Ordered differential display (ODD) was used to clone differentially expressed genes in rice induced by Magnaporthe grisea. Of 37 cDNA clones initially identified by reverse Northern blot, 5 clones were chosen as probes for further Northern test. The result showed that all of these 5 clones were expressed both in highly, moderately resistant and susceptible rice lines in a pathogen inducible manner. According to the homology analysis, these clones were suggested to be involved in plant defense response by: (1) inhibiting growth of fungus and even causing its death; (2) excluding toxic compounds delivered by fungus; (3) priming signal transduction; (4) and/or regulating metabolic activities in agreement with plant defense.