Defense Proteins from Vigna Unguiculata
Seed Exudates: Characterization and Inhibitory Activity Against Fusarium Oxysporum |
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Authors: | Tatiana L Rose Alexandre da Silva Conceição Jose Xavier-Filho Lev A Okorokov Kátia V S Fernandes Francis Marty Danièle Marty-Mazars André O Carvalho Valdirene M Gomes |
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Institution: | 1. Laboratório de Fisiologia e Bioquímica de Microrganismos, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Av. Alberto Lamego 2000, 28013-600, Campos dos Goytacazes, RJ, Brazil 2. Laboratoire de PhytoBiologie Cellulaire, Université de Bourgogne, BP 47870, 21078, Dijon Cedex, France 3. Laboratório de Química e Fun??o de Proteínas e Peptídeos, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Av. Alberto Lamego 2000, 28013-600, Campos dos Goytacazes, RJ, Brazil
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Abstract: | Plants exude a variety of substances through their roots, germinating seeds and aerial parts. Some of these released compounds seem to have an inhibitory effect against pathogens. The aim of this work was to investigate and identify antifungal proteins present in exudates from imbibed cowpea seeds (Vigna unguiculata (L.) Walp). The obtained exudation was analyzed in regard to specific protein activities by enzymatic or immunological assays for plant defense proteins, from 4 h to 48 h of seed imbibition. Our results show that cowpea seeds exudates present several defense related proteins characterized as β-1,3-glucanases, cystatins, vicilins and lipid transfer proteins (LTPs), as well as a storage vacuole membrane α-TIP protein, since the very first hours of imbibition. These exudates also have an “in vitro” inhibitory effect on the growth of the fungus Fusarium oxysporum f. sp. phaseoli. Our results suggest that seed exudates should promote seed protection from soil pathogens. |
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Keywords: | LTP β -1 3-glucanases Vicilin Cystatin Fusarium oxysporum f sp phaseoli Seed exudates Soil pathogen Cowpea |
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