大鳞副泥鳅粗线期二价染色体分带研究

报道了一种鱼类二价体显带的新,采用ＰＨ值在７．２－７．４之间的低涌液对大鳞副泥鳅精巢进行整体长低渗、卡诺固定液处理方法得到粗线期二阶染色体的高分辨Ｇ带，其带纹特征和数目相对稳定且清晰可辨。与胰酶显和ＥＤＴＡ显相比具有操作简单，耗时短以及二价体分散相对较好等特点，这一方法在所有显带方法中最为简便。

Pachytene Chromosome Banding of Paramisgurnus dabryanus

A new method for Pachytene chromosome G-banding was reported in this paper.The whole testis was treated with basic hypotonic solution(pH 7.2～7.4) for 4～6 h,then treated with Carnoy's solution for 3～4 times.The cell suspensions were discarded on cold slides.The high-resolution G-bands of meiotic pachytene chromosome in Paramisgurnus dabryanus was obtained after dyed with Giemsa.The characteristic and numbers of bands were stable and distinct.Compared with trypsin or EDTA-treated methods,this protocol is the simplest one with operation-simple,less time-needed and high rate of success.