Stability of a recombinant plasmid carrying a-amylase gene in Bacillus subtilis |
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Authors: | Gu¨rakan G.C. Bayındırlı A. O¨gel Z.B. Tanbogğa P. |
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Affiliation: | 1. Department of Food Engineering, Middle East Technical University, 06531, Ankara, Turkey ; fax
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Abstract: | Plasmid pSB6 is a streptococcal recombinant plasmid carrying the a-amylase gene of Bacillus amyloliquefaciens and the chloramphenicol resistance gene. The segregational and structural instabilities of this plasmid were examined under non-selective conditions in Bacillus subtilis. These instabilities were modelled according to a kinetic expression derived from the difference in the growth between plasmid-bearing and plasmid-free cells. This plasmid showed slight segregational instability and much higher levels of structural instability under the conditions examined. |
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